Original Trials to Increase Penis Size Safely

Details of Trials

Patients & Indications

Upon completing his training with Professor Sava Perovic from May to September 2003, Dr Kim Jin Hong MD PhD returned to Seoul to bring the human trials of penile girth enhancement of Dr Jae Sang Byun MD to Europe in 2004.

Permanent penis enlargement surgery by Professor Sava Perovic and Dr Kim Jin Hong MD PhD

A total of 84 men ranging in age from 20 to 50 years (average age 28 years) were randomly selected to participate in the study from the 204 patients who obtained penile girth enhancement by tissue engineering from July 1999 to early 2004 from Dr Byun's Yeonsei-Plus Urologic Clinic in Seoul, the University Hospital in Belgrade and finally the Presidential Hospital of Russia in Moscow.

Among those selected, 70.24% suffered penile dysmorphic disorder (preoccupation with an imagined problem in penile appearance) while 25 patients (29.76%) had a history of previously failed penile girth enlargement.

Exclusion criteria were:

  • a) history of previous psychiatric morbidity;
  • b) organic diseases (e.g.; long-lasting, insulin-dependent Diabetes Mellitus and generalized atherosclerosis); and
  • c) age less than 18 years.

Physical measurements of penile length and mid-shaft penile circumference in both the flaccid and erect states were obtained and recorded pre and post-operatively.

Pre-operative and post-operative intracavernous injection of Prostaglandine E1 and Doppler ultrasound were used for assessment of erectile function.

Cell Harvest Method

Dr Jae Sang Byun, Professor Sava Perovic, Dr Kim Jin Hong

After local infiltration of 1% of lidocaine solution, a thumbnail sized, elliptical shaped skin incision was made and 0.5–1.0 cm3 of dermal tissue was harvested.

Biopsied dermal tissue was washed to remove red blood cells in serum-free Dulbecco’s modified eagle’s medium and minced into less then 1 mm pieces which were then distributed over three 100 x 15 mm Petri dishes.

The sliced tissues were treated with collagenase type IV, (2 mg/ml) and incubated at 37.8°C with 5% carbon dioxide for 24 hours.

Fibroblasts were suspended in culture media containing 5% of the patient’s serum and expanded in culture until the total cell number of at least 2 x 107 was reached.

The time required was between three and five weeks depending upon individual patients (mean 27 days).

This method has been surpassed by a superior technique in which a small amount of the patient's own blood is taken the day before surgery.

Serum is produced from that and used to prepare the scaffolds. (No longer is dermal tissue biopsied unless specifically requested by the patient.)

Scaffold Pre-Treatment and Cell Seeding

Permanent penis enlargement surgery by Sava Perovic Foundatio

Dry Korean polylactic-co-glycolic acid scaffold (PLGA) 50 mm in length, 30 mm in inner diameter and 3 mm in thickness, pore size 250 to 400 mm, was hydrated by complete immersion into 75% ethanol solution in aseptic condition and kept in a refrigerator (4°C) overnight.
[Also know as a Poly(Lactic-co-Glycolic Acid) polymeric scaffold.]

Starting in 2008, Sava Perovic began using 4 mm thick scaffolds “because all patients want more girth enhancement, but we also still have the thinner ones (3 mm).”

Some patients prefer to restrain their girth increase and choose the thinner PLGA scaffolds.

The scaffolds are a standard thickness so the percentage of net girth increase varies depending on the original circumference of the penis.

Smaller penises will experience a greater “percentage” of increase in girth. Larger penises will enjoy the same girth increase in real terms but a smaller “percentage”.

The ethanol solution was removed completely by repeated washing with sterile, pyrogen-free cold water, phosphate-buffered saline solution (Ca, Mg-free, pH 7.0) and serum-free culture medium.

Pretreated scaffolds were then seeded with approximately 20 million fibroblasts and incubated for 24 hours at 37°C.


Prophylactic antibiotic administration was systematically prescribed 24 hours before surgery.

Surgical penis enlargement photo: Penis enlargement pics - penis enlargement before and after

After subcoronal incision and penile degloving, two cell-seeded scaffolds were shape adjusted and transplanted between dartos and Buck’s fascia.

When the skin was non-compliant, scaffolds were placed under the lifted neurovascular bundle.

A ventral longitudinal incision with penile inversion and degloving was used in patients who requested prepuce (foreskin) preservation.

Penile skin reconstruction followed repair of dartos fascia.

A compressive dressing was left in place for 7–10 days.

Antibiotics were administrated for the next five days.

Two weeks after surgery, patients started to use a vacuum device twice a day (5–10 minutes) during the first six months to prevent temporary penile retraction.

The clinical data concerning the final outcome and complications were obtained and recorded on control visits at two weeks as well as at 1, 3, 6 & 12 months after surgery and then annually.


Autologous tissue engineering is the best penis enlargement surgery because it allows uncircumcised patients to keep their foreskin intact

A study instrument comprised of a short, structured questionnaire, was modified and adopted from a validated study for a long-term outcome evaluation in hypospadias surgery.

Answer options ranged from dissatisfied and somewhat satisfied to completely satisfied.

Surgery was also judged on a scale from 1 to 5 with 5 being the best.

A genital appraisal was assessed in the second part of the questionnaire.

Possible answer options for the patients-rated satisfaction with flaccid and erect penile girth were “satisfied” or “dissatisfied” while scores for satisfaction with general penile appearance ranged from 1 to 5 on the following scale:

  • 1 = very dissatisfactory;
  • 2 = dissatisfactory;
  • 3 = good;
  • 4 = very good; and
  • 5 = excellent.

Data analysis was done using Students t-test (t-test for dependent samples), the X2 test with Yates correction (Yates corrected Chi-square).

A p value of 0.5, 0.01 and 0.001 were considered statistically significant. next:  Results

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